Hydroxyapatite - Hyaluronic acid scaffold production using solvent extraction method

Document Type : Research Paper

Authors

• arash yazdani ¹
• Armaghan Noorbakhsh ²

¹ دانشگاه صنعتی شاهرود، دانشکده مهندسی شیمی و مواد

² School of Chemistry and Materials Enginnering, Shahrood University of Technology

Abstract

The purpose of this study was to evaluate the simultaneous effect of Hyaluronic acid (HA) - Polyvinyl alcohol (PVA) - Gelatin (Gel), and nano Hydroxyapatite (nHAp), which is mineral in bone. In this study, for the first time, bone scaffold of nHAp and bioresorbable polymers (HA, PVA, and Gel) was prepared by solvent evaporation method. The composite specimens were prepared using nHAp-PVA, nHAp-PVA-Gel and nHAp-PVA-HA, four-component nHAp-PVA-HA-Gel, using a simple liquidation process. Phase and chemical analysis were investigated by FTIR and XRD tests. The distribution and porosity size and their relationship to each other as well as the roughness of porosity were studied using scanning electron microscopy. The compressive strength test was also performed on tablets prepared from this composite. The FTIR test did not indicate the formation of a new chemical compound. The compressive strength indicated that the four minor sample had the highest numerical value. Investigating the SEM analysis of the formation of scaffolds for a four-part sample, which shows porosity percentage, porosity size and bonding, suggest the suitability of this sample for tissue engineering applications. The analysis of the XRD is an amorphous specimen and, given the similarity of the natural bone XRD diagram, this composite can be used as a bone filler.

Keywords

• Scaffold
• Hyaluronic acid
• PVA
• Hydroxyapatite
• Solvent Evaporation

20.1001.1.22285946.1398.9.36.10.4

---

References

References:
[1]        E. Kon et al., “Autologous bone marrow stromal cells loaded onto porous hydroxyapatite ceramic accelerate bone repair in critical‐size defects of sheep long bones,” J. Biomed. Mater. Res. An Off. J. Soc. Biomater. Japanese Soc. Biomater., vol. 49, no. 3, pp. 328–337, 2000.   

[2]        حیدری, “ساخت و مقایسه دار بست های هیدروکسی آپاتیت طبیعی و مصنوعی ساخته شده به روش فشار ایزواستاتیک سرد,” فصلنامه علمی-پژوهشی مواد نوین, vol. 9, no. 33, pp. 113–124, 2018.

[3]        م. مظفری, ن. جوهری, and م. ح. فتحی, “داربست کامپوزیتی پلی‌کاپرولاکتون-هیدروکسی آپاتیت: بررسی تاثیر درصد ذرات هیدروکسی آپاتیت و مقایسه ذرات با سایز نانومتری و میکرومتری و اثر آن‌ها بر خواص مکانیکی و زیست‌تخریب‌پذیری داربست,” فصلنامه علمی-پژوهشی مواد نوین, vol. 5, no. 20, pp. 131–142, 2015.    

[4]        J. C. Doadrio, D. Arcos, M. V Cabanas, and M. Vallet-Regı, “Calcium sulphate-based cements containing cephalexin,” Biomaterials, vol. 25, no. 13, pp. 2629–2635, 2004.    

[5]        N. J. Kaljahi, B. Ghanbarzadeh, J. Dehghannya, and A. Akbar, “Plasticized Starch Based Bionanocomposites

Containing Cellulose Nanowhiskers and Titanium Dioxide Nanoparticles: Study of Structure and Water Vapor Permeability,” Sci. Technol., vol. 27, no. 3, pp. 179–192, 2014.

[6]        Y. Pan and D. Xiong, “Study on compressive mechanical properties of nanohydroxyapatite reinforced poly (vinyl alcohol) gel composites as biomaterial,” J. Mater. Sci. Mater. Med., vol. 20, no. 6, pp. 1291–1297, 2009.   

[7]        G. Kogan, L. Šoltés, R. Stern, and P. Gemeiner, “Hyaluronic acid: a natural biopolymer with a broad range of biomedical and industrial applications,” Biotechnol. Lett., vol. 29, no. 1, pp. 17–25, 2007.

[8]        D. Bakoš, M. Soldan, and I. Hernandez-Fuentes, “Hydroxyapatite–collagen–hyaluronic acid composite,” Biomaterials, vol. 20, no. 2, pp. 191–195, 1999.

[9]        F. Wang, E. Guo, E. Song, P. Zhao, and J. Liu, “Structure and properties of bone-like-nanohydroxyapatite/gelatin/polyvinyl alcohol composites,” Adv. Biosci. Biotechnol., vol. 1, no. 03, p. 185, 2010.

[10]      S. Subramaniam, Y.-H. Fang, S. Sivasubramanian, F.-H. Lin, and C. Lin, “Hydroxyapatite-calcium sulfate-hyaluronic acid composite encapsulated with collagenase as bone substitute for alveolar bone regeneration,” Biomaterials, vol. 74, pp. 99–108, 2016.       

[11]      معروف et al., “بررسی خواص فیزیکوشیمیایی داربست کامپوزیتی کیتوسان-ژلاتین-هیدروکسی آپاتیت تهیه شده به روش خشکاندن انجمادی,” مجله دانشکده دندانپزشکی دانشگاه علوم پزشکی شهید بهشتی, vol. 29, no. 5, pp. 390–398, 2012.